供應“Recombinant Murine IL-12”價格
Catalog Number:
210-12
Synonyms:
NKSF, CTL maturation factor （TCMF）, Cytotoxic lymphocyte maturation factor （CLMF）, TSF
Description:
IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-γ production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue type mast cells. Among its biological activities IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells and induces the development of IFN-γ producing Th1 cells. Recombinant murine IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa （p35） and 40 kDa （p40） subunits （506 total amino acid residues）.
AA Sequence:
p35 Subunit:RV*SG PARCLSQSRN LLKTTDDMVK TAREKLKHYS CTAEDIDHED ITRDQTSTLK TCLPLELHKN ESCLATRETS STTRGSCLPP QKTSLMMTLC LGSIYEDLKM YQTEFQAINA ALQNHNHI ILDKGMLVAI DELMQSLNHN GETLRQKPPV GEADPYRVKM KLCILLHAFS TRVVTINRVM GYLSSA
p40 Subunit:MWELEKD VYVVEVDWTP DAPGETVNLT CDTPEEDDIT WTSDQRHGVI GSGKTLTITV KEFLDAGQYT CHKGGETLSH SHLLLHKKEN GIWSTEILKN FKNKTFLKCE APNYSGRFTC SWLVQRNMDL KFNIKSSSSS PDSRAVTCGM ASLSAEKVTL DQRDYEKYSV SCQEDVTCPT AEETLPIELA LEARNKYE NYSTSFFIRD IIKPDPPKNL QMKPLKNSQV EVSWEYPDSW STPHSYFSLK FFVRIQRKKE KMKETEEGCN QKGAFLVEKT STEVQCKGGN VCVQAQDRYY NSSCSKWACV PCRVRS
Source:
CHO cells
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity:
Assay #1: The ED50 was determined by the stimulation of IFN-gamma production by murine splenocytes co-stimulated with IL-18 is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg.
Assay #2: Determined by its ability to stimulate the proliferation of 2D6 cells. The expected ED50 is ≤0.1ng/ml, corresponding to a specific activity of ≥1 x 107 units/mg.
Assay #2: Determined by its ability to stimulate the proliferation of 2D6 cells. The expected ED50 is ≤0.1ng/ml, corresponding to a specific activity of ≥1 x 107 units/mg.
Cross Reactivity:
Human, Mouse, N/A, Cow + Mouse
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