Sfi I from Streptomyces fimbriatus1,250 U (10 U/μl)(Roche) 編號(hào):11288024001
免費(fèi)代測(cè)ELISA試劑盒一周內(nèi)出結(jié)果
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主營(yíng)產(chǎn)品: ELISA試劑盒,生物試劑,免疫組學(xué),生物抗體,蛋白組學(xué),分子生物等。
Special incubation temperature: 50°C
Product Description
Heat inactivation: Sfi I cannot be heat inactivated by 15 min incubation at 65°C.
Compatible ends: For an Sfi I end to be compatible and ligatable with any other end, the two corresponding recognition sites must have contained the same three internal Ns.
Unit definition: One unit is the enzyme activity that compley cleaves 1 µg Ad2 DNA in 1 h at 50°C in a total volume of 25 µl SuRE/Cut buffer M.
Application
Isoschizomers: The enzyme has no known isoschizomers. Because of its unusual recognition sequence, Sfi I is useful for analyzing and cloning large DNA fragments. Methylation sensitivity: Sfi I is inhibited by the presence of 5-methylcytosine at the most central C residue (*) on the recognition sequence. 5-methylcytosine at the other C-positions (°) will not inhibit the enzyme.了解更多詳細(xì)信息
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