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　　人布魯氏菌抗體IgGELISA檢測(cè)試劑盒屬固相免疫測(cè)定，抗原、抗體的結(jié)合只在固相表面上發(fā)生。以抗體包被的夾心法為例，加入板孔中的標(biāo)本，其中的抗原并不是都有均等的和固相抗結(jié)合的機(jī)會(huì)，只有zui貼近孔壁的一層溶液中的抗原直接與抗體接觸。

　　這是一個(gè)逐，因此需經(jīng)擴(kuò)散才能達(dá)到反應(yīng)的終點(diǎn)。在其后加入的酶標(biāo)記抗體與固相抗原的結(jié)合也同樣如此。這就是為什么ELISA檢測(cè)試劑盒反應(yīng)總是需要一定時(shí)間的溫育。

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　　溫育人布魯氏菌抗體IgGELISA檢測(cè)試劑盒常采用的溫度有43℃、37℃、室溫和4℃（冰箱溫度）等。37℃是實(shí)驗(yàn)室中常用的保溫溫度，也是大多數(shù)抗原抗體結(jié)合的合適溫度。在建立ELISA方法作反應(yīng)動(dòng)力學(xué)研究時(shí)，實(shí)驗(yàn)表明，兩次抗原抗體反應(yīng)一般在37℃經(jīng)1-2小時(shí)，產(chǎn)物的生成可達(dá)。

中文名稱：大人布魯氏菌抗體IgGELISA檢測(cè)試劑盒
規(guī)    格：48孔/人份、96孔/人份，全部為批次
特 異 性：本試劑盒可同時(shí)檢測(cè)天然或重組的，且與其他相關(guān)蛋白無(wú)交叉反應(yīng)
特    點(diǎn)：靈敏性高、特異性強(qiáng)、重復(fù)性好
供 應(yīng) 商：上海哈靈生物科技有限公司

　　為加速反應(yīng)，可提高人布魯氏菌抗體IgGELISA檢測(cè)試劑盒反應(yīng)的溫度，有些試驗(yàn)在43℃進(jìn)行，但不宜采用更高的溫度?？乖贵w反應(yīng)4℃更為*，在放射免疫測(cè)定中多使反應(yīng)在冰箱中，以形成zui多的沉淀。但因所需時(shí)間太長(zhǎng)，在ELISA試劑盒中一般不予采用。

　　人布魯氏菌抗體IgGELISA檢測(cè)試劑盒保溫的方式除有的ELISA檢測(cè)試劑盒儀器附有特制的電熱塊外，一般均采用水浴，可將ELISA試劑盒板置于水浴箱中，ELISA檢測(cè)試劑盒板底應(yīng)貼著水面，使溫度迅速平衡。為避免蒸發(fā)，板上應(yīng)加蓋，也可用塑料貼封紙或保鮮膜覆蓋板孔，此時(shí)可讓反應(yīng)板漂浮在水面上。

　　若用保溫箱，人布魯氏菌抗體IgGELISA檢測(cè)試劑盒板應(yīng)放在濕盒內(nèi)，濕盒要選用傳熱性良好的材料如金屬等，在盒底墊濕的紗布，zui后將ELISA試劑盒板放在濕紗布上。濕盒應(yīng)先放在保溫箱中預(yù)溫至規(guī)定的溫度，特別是在氣溫較低的時(shí)候更應(yīng)如此。無(wú)論是水浴還是濕盒溫育，反應(yīng)板均不宜疊放，以保證各板的溫度都能迅速平衡。

　　室溫溫育的反應(yīng)，人布魯氏菌抗體IgGELISA檢測(cè)試劑盒操作時(shí)的室溫應(yīng)嚴(yán)格限制在規(guī)定的范圍內(nèi)，標(biāo)準(zhǔn)室溫溫度是指20-25℃，但具體操作時(shí)可根據(jù)說(shuō)明書的要求控制溫育。  室溫溫育時(shí)，ELISA檢測(cè)試劑盒板只要平置于操作臺(tái)上即可。應(yīng)注意溫育的溫度和時(shí)間應(yīng)按規(guī)定力求準(zhǔn)確。為保證這一點(diǎn)，一個(gè)人操作時(shí)，一次不宜多于兩塊板同時(shí)測(cè)定。

人布魯氏菌抗體IgGELISA檢測(cè)試劑盒相關(guān)細(xì)胞：

293T/17HLX3001DMEM+10%FBS人胚腎細(xì)胞
"RT4HLX3002McCOY's 5+10%FBS
人膀胱移行細(xì)胞乳頭瘤細(xì)胞"
"BHK-21 [C-13]HLX3003MEM培養(yǎng)基10%FBS
倉(cāng)鼠腎成纖維細(xì)胞"
CHLHLX3004DMEM培養(yǎng)基+10%FBS倉(cāng)鼠肺細(xì)胞
"V79HLX3005
 RPMI-1640(GIBCO）+20%FBS
 倉(cāng)鼠肺細(xì)胞"
R 1610HLX3006DMEM+10%FBS倉(cāng)鼠肺細(xì)胞
CHO/dhFr-HLX3007IMDM培養(yǎng)基(GIBCO)+10%FBS倉(cāng)鼠卵巢細(xì)胞,二氫葉酸還原酶缺陷
CHO-K1HLX3008F-12K+10%FBS倉(cāng)鼠卵巢細(xì)胞亞株
"Lec1HLX3009MEMα培養(yǎng)基(GIBCO)+10%FBS
倉(cāng)鼠卵巢細(xì)胞"
CTLA4 Ig-24HLX3010DMEM培養(yǎng)基+10%FBS中國(guó)倉(cāng)鼠卵巢細(xì)胞
L6HLX3011DMEM培養(yǎng)基+10%FBS大鼠成肌細(xì)胞
"NR8383HLX3012F12K培養(yǎng)基(SIGMA)+20%FBS
大鼠肺泡巨噬細(xì)胞"
H9c2(2-1)HLX3013DMEM+10%FBS+1%P/S大鼠心肌細(xì)胞
RH-35HLX3014DMEM培養(yǎng)基+10%FBS大鼠肝癌細(xì)胞
BRLHLX3015DMEM+10%FBS大鼠肝細(xì)胞
BRL 3AHLX3016DMEM培養(yǎng)基+10%FBS大鼠肝細(xì)胞
C6HLX3017DMEM+10% FBS大鼠膠質(zhì)瘤細(xì)胞
SHZ-88HLX3018DMEM培養(yǎng)基+10%FBS大鼠乳腺癌細(xì)胞
Y3-Ag 1.2.3HLX3019DMEM培養(yǎng)基+10%FBS大鼠骨髓瘤細(xì)胞
UMR-106HLX3020DMEM培養(yǎng)基+10%FBS大鼠骨肉瘤細(xì)胞
PC-12(高分化)HLX3021RPMI-1640(GIBCO）+10%FBS大鼠腎上腺嗜鉻細(xì)胞瘤細(xì)胞(高分化)
NRKHLX3022DMEM培養(yǎng)基+10%FBS大鼠腎細(xì)胞
NRK-52EHLX3023DMEM培養(yǎng)基+5%FBS大鼠腎細(xì)胞
"RBL-2H3HLX3024
 MEM培養(yǎng)基(GIBCO)+15%熱滅活FBS
 大鼠嗜堿性細(xì)胞白血病細(xì)胞"
RSC96HLX3025DMEM+10%FBS大鼠雪旺細(xì)胞
Mv.1.Lu(NBL-7)HLX3026MEM培養(yǎng)基(GIBCO)+10%FBS貂肺上皮細(xì)胞
COS-1HLX3027DMEM培養(yǎng)基+10%FBS非洲綠猴SV40轉(zhuǎn)化的腎細(xì)胞
COS-7HLX3028DMEM培養(yǎng)基+10%FBS非洲綠猴SV40轉(zhuǎn)化的腎細(xì)胞
CV-1 [Part of the Wistar Special Collection]HLX3029RPMI-1640(GIBCO）+10%FBS非洲綠猴腎細(xì)胞
VeroHLX3030MEM培養(yǎng)基(GIBCO)+10%FBS非洲綠猴腎細(xì)胞
VERO C1008 (E6)HLX3031DMEM培養(yǎng)基+10%FBS非洲綠猴腎細(xì)胞
FRhK-4HLX3032DMEM培養(yǎng)基+10%FBS恒河猴胚腎細(xì)胞
LLC-MK2HLX3033RPMI-1640(GIBCO）+10%FBS恒河猴腎細(xì)胞
RF/6AHLX3034RPMI-1640(GIBCO）+10%FBS猴脈絡(luò)膜-視網(wǎng)膜(內(nèi)皮)細(xì)胞
B95-8HLX3035RPMI-1640(GIBCO）+10%FBS絨猴EBV轉(zhuǎn)化的白細(xì)胞
OKHLX3036MEM培養(yǎng)基(GIBCO)+10%FBS負(fù)鼠腎細(xì)胞
MDCK(NBL-2)HLX3037MEM(GIBCO)+10%FBS狗腎細(xì)胞
"Super TubeHLX3038D-MEM/F-12培養(yǎng)基(GIBCO)+10%FBS
狗腎細(xì)胞"
UMNSAH/DF-1HLX3039DMEM培養(yǎng)基+10%FBS雞胚成纖維細(xì)胞
DH82HLX3040MEM培養(yǎng)基(GIBCO)+10%FBS狗腎惡性組織細(xì)胞增生癥細(xì)胞
CRFKHLX3041RPMI-1640(GIBCO）+10%FBS貓腎細(xì)胞
F81HLX3042RPMI-1640(GIBCO）+10%FBS貓腎細(xì)胞
EBTr (NBL-4)HLX3043MEM培養(yǎng)基(GIBCO)+10%FBS牛胚氣管細(xì)胞
MDBK (NBL-1)HLX3044RPMI-1640(GIBCO）+10%FBS牛腎細(xì)胞
Pt K1 (NBL-3)HLX3045MEM培養(yǎng)基(GIBCO)+10%FBS袋鼠腎細(xì)胞
PIECHLX3046RPMI-1640(GIBCO）+10%FBS豬髖動(dòng)脈內(nèi)皮細(xì)胞
NIH/3T3HLX3047DMEM+10% FBS小鼠胚胎細(xì)胞
NIH/3T3HLX3047DMEM+10%NBCS(小牛血清）小鼠胚胎細(xì)胞
3T6-Swiss albinoHLX3048DMEM培養(yǎng)基+10%FBS小鼠胚胎成纖維細(xì)胞
BALB/3T3 clone A31HLX3049DMEM培養(yǎng)基+10%FBS小鼠胚胎成纖維細(xì)胞
C3H/10T1/2, Clone 8HLX3050MEM培養(yǎng)基(GIBCO)+10%FBS小鼠胚胎成纖維細(xì)胞
Psi2 DAPHLX3051DMEM培養(yǎng)基+10%FBS小鼠胚胎成纖維細(xì)胞
C2C12HLX3052DMEM+10%FBS小鼠成肌細(xì)胞
A9HLX3053DMEM培養(yǎng)基+10%FBS小鼠皮下結(jié)締組織細(xì)胞
L Wnt-3AHLX3054DMEM培養(yǎng)基+10%FBS小鼠皮下結(jié)締組織細(xì)胞
NCTC clone 929 [L cell, L-929, derivative of Strain L]HLX3055MEM(NaHCO3 1.5g/L, Sodium Pyruvate 0.11g/L)+10%FBS小鼠成纖維細(xì)胞
B16HLX3056RPMI-1640(GIBCO）+10%FBS小鼠黑色素瘤細(xì)胞
B16-F10HLX3057DMEM培養(yǎng)基+10%FBS小鼠皮膚黑色素瘤細(xì)胞
MFCHLX3058RPMI-1640(GIBCO）+10%FBS小鼠胃癌細(xì)胞
BNL CL.2HLX3059DMEM培養(yǎng)基+10%FBS小鼠胚胎肝細(xì)胞
MS1HLX3060DMEM培養(yǎng)基+5%FBS小鼠胰島內(nèi)皮細(xì)胞
Beta-TC-6HLX3061DMEM培養(yǎng)基+15%熱滅活FBS小鼠胰島素瘤胰島β細(xì)胞
CT26.WTHLX3062DMEM培養(yǎng)基+10%FBS小鼠結(jié)腸癌細(xì)胞
K7M2 wt [K7M2-WT]HLX3063DMEM培養(yǎng)基+10%FBS小鼠骨肉瘤成骨細(xì)胞
Hepa 1-6HLX3064DMEM+10%FBS小鼠肝癌細(xì)胞
"MC3T3-E1 Subclone 14HLX306-MEM培養(yǎng)基+10%FBS
小鼠顱頂前骨細(xì)胞亞克隆14"
"OP9HLX3066a-MEM培養(yǎng)基+20%FBS
小鼠骨髓基質(zhì)細(xì)胞"
RAGHLX3067MEM培養(yǎng)基(GIBCO)+10%FBS小鼠腎腺癌細(xì)胞
SV40 MES 13HLX3068DMEM培養(yǎng)基+5%FBS小鼠腎小球系膜細(xì)胞
Y1HLX3069RPMI-1640(GIBCO）+10%FBS小鼠腎上腺皮質(zhì)瘤細(xì)胞
P815HLX3070DMEM培養(yǎng)基+10%FBS小鼠肥大細(xì)胞瘤細(xì)胞
RM-1HLX3071RPMI-1640(GIBCO）+10%FBS小鼠前列腺癌細(xì)胞
MLTC-1HLX3072RPMI-1640(GIBCO）+10%FBS小鼠睪丸間質(zhì)細(xì)胞瘤細(xì)胞
"TM3HLX3073D-MEM/F-12培養(yǎng)基(GIBCO)+5%馬血清+2.5%FBS
小鼠睪丸間質(zhì)細(xì)胞"
F9HLX3074DMEM培養(yǎng)基+10%FBS小鼠畸胎瘤細(xì)胞
P19HLX307-MEM（肌醇 43.2mg/l,葉酸 8.82mg/l)+7.5%BCS+2.5%FBS小鼠畸胎瘤細(xì)胞
4T1HLX3076RPMI-1640(GIBCO）+10%FBS小鼠乳腺癌細(xì)胞
C127HLX3077DMEM培養(yǎng)基+10%FBS小鼠乳腺腫瘤細(xì)胞
LLCHLX3078DMEM+10%FBS小鼠Lewis肺癌細(xì)胞
S-180HLX3079RPMI-1640(GIBCO）+10%FBS小鼠腹水瘤細(xì)胞
Neuro-2a [N2a; Neuro-2a]HLX3080MEM培養(yǎng)基(GIBCO)+10%FBS小鼠腦神經(jīng)瘤細(xì)胞
AtT-20HLX3081RPMI-1640(GIBCO）+10%FBS小鼠垂體瘤細(xì)胞
FOHLX3082DMEM培養(yǎng)基+10%FBS小鼠骨髓瘤細(xì)胞
P3/NSI/1-Ag4-1 [NS-1]HLX3083DMEM培養(yǎng)基+10%FBS小鼠骨髓瘤細(xì)胞
P3X63Ag8HLX3084DMEM培養(yǎng)基+10%FBS小鼠骨髓瘤細(xì)胞
P3X63Ag8.653HLX3085RPMI-1640(GIBCO）+10%FBS小鼠骨髓瘤細(xì)胞
SP2/0HLX3086RPMI-1640(GIBCO）+10%FBS小鼠骨髓瘤細(xì)胞
"P388D1HLX3087RPMI-1640(GIBCO）+90%馬血清+10%FBS
小鼠淋巴樣瘤細(xì)胞"
EL4HLX3088RPMI-1640(GIBCO）+10%FBS小鼠淋巴瘤細(xì)胞
YAC-1HLX3089RPMI-1640(GIBCO）+10%FBS小鼠淋巴瘤細(xì)胞
L5178Y TK+/- clone (3.7.2C)HLX3090DMEM培養(yǎng)基+10%FBS小鼠淋巴瘤細(xì)胞
Ana-1HLX3091RPMI-1640(GIBCO）+10%FBS小鼠巨噬細(xì)胞
RAW 264.7HLX3092DMEM+10% FBS小鼠單核巨噬細(xì)胞白血病細(xì)胞
L1210HLX3093DMEM培養(yǎng)基+10%FBS小鼠白血病細(xì)胞
L6565HLX3094RPMI-1640(GIBCO）+10%FBS小鼠白血病克隆細(xì)胞系
3T3-L1HLX3095DMEM+10%FBS小鼠胚胎成纖維細(xì)胞
A-431HLX3096DMEM+10%FBS人表皮癌細(xì)胞
HT-1080HLX3097MEM培養(yǎng)基(GIBCO)+10%FBS人纖維肉瘤
"KBHLX3098MEM培養(yǎng)基(GIBCO）+10%FBS
RPMI-1640(GIBCO）+10%FBS
人口腔表皮樣癌細(xì)胞"
FaDuHLX3099MEM(GIBCO)+10%FBS人咽鱗癌細(xì)胞
"TTHLX3100F-12K+10%FBS
人甲狀腺導(dǎo)管癌細(xì)胞"
"SW579 [SW 579; SW-579]HLX3101L-15培養(yǎng)基+10%FBS
人甲狀腺鱗癌細(xì)胞"
TE-1HLX3102RPMI-1640(GIBCO）+10%FBS人食管癌細(xì)胞
NCI-N87 [N87]HLX3103RPMI-1640(GIBCO）+10%FBS人胃癌細(xì)胞
MGC80-3HLX3104RPMI-1640(GIBCO）+10%FBS人胃癌細(xì)胞
HGC-27HLX3105RPMI-1640(GIBCO）+20%FBS人胃癌細(xì)胞（未分化）
AGSHLX3106F-12+10% FBS人胃腺癌細(xì)胞
SGC-7901HLX31071640+10% FBS人胃腺癌細(xì)胞
BGC-823HLX31081640+10% FBS人胃腺癌細(xì)胞（低分化）
BEL-7402HLX31091640+10% FBS 人肝癌細(xì)胞
BEL-7404HLX31101640+10% FBS 人肝癌細(xì)胞
Hep 3B2.1-7HLX3111MEM培養(yǎng)基(GIBCO)+10%FBS人肝癌細(xì)胞
Hep G2HLX3112DMEM+10% FBS人肝癌細(xì)胞
SK-HEP-1HLX3113MEM(GIBCO)+10%FBS人肝癌細(xì)胞
SMMC-7721HLX3114DMEM+10% FBS人肝癌細(xì)胞
PLC/PRF/5HLX3115MEM培養(yǎng)基(GIBCO)+10%FBS人肝癌亞力山大細(xì)胞
Li-7HLX31161640+10%FBS人肝癌細(xì)胞
HuH-7HLX3117DMEM+10% FBS人肝癌細(xì)胞
HuH-6HLX3118DMEM+10% FBS人肝母細(xì)胞瘤細(xì)胞
RBEHLX31191640+10% FBS人肝膽管癌細(xì)胞
MRC-5HLX3120MEM+10% FBS人胚肺細(xì)胞
WI-38HLX3121MEM培養(yǎng)基(GIBCO)+10%FBS人胚肺細(xì)胞
THLE-3HLX3122MD 21793的BEGM培養(yǎng)基(目錄號(hào)CC3170)人肝永生化細(xì)胞
HCCC-9810HLX3123RPMI-1640(GIBCO）+10%FBS人膽管細(xì)胞型肝癌細(xì)胞
GBC-SDHLX3124RPMI-1640(GIBCO）+10%FBS人膽囊癌細(xì)胞
"CFPAC-1HLX3125IMDM(GIBCO）+10%FBS
PANC-1HLX3126DMEM+10% FBS人胰腺癌細(xì)胞
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AsPC-1HLX31291640+10% HLXlone滅活血清人轉(zhuǎn)移胰腺腺癌細(xì)胞