雙染蛋白MARKERPrecision Plus Protein™ Dual Color Stand
Precision Plus Protein™ Dual Color Standards #161-0374
500 μl, mixture of 10 recombinant proteins （10–250 kD）, 8 blue-stained bands, and 2 pink reference bands （25 and 75 kD）, 50 applications
- Description
- 技術(shù)指標(biāo)
| MW size range, kD | 10–250 |
| Stain type | Dual Color |
| Volume, µl | 500 |
| Total number of applications | 50 |
| Shelf life at –20°C, months | 12 |
| Loading volume on mini gels, µl | for Coomassie staining, 10 |
| Loading volume on mini gels, µl | for fluorescent blot detection, 5 of 1:10 dilution |
| Application Type | |
| Monitoring electrophoretic separation for SDS-PAGE gels | Yes |
| Molecular weight sizing on SDS-PAGE gels | Yes |
| Monitoring blot transfer efficiency | Yes |
| Fluorescence blots | Yes |
| Molecular weight sizing on | N/A |
| Loading Volume on Mini Gels | |
| For Coomassie staining, µl | N/A |
| For stain-free gels, µl | N/A |
| For silver staining, µl | N/A |
| For SYPRO Ruby staining, µl | N/A |
| For blot development, µl | N/A |
| StrepTactin conjugate working dilutions for colorimetric blot development | N/A |
| StrepTactin conjugate working dilutions for chemiluminescence blot development | N/A |
The Precision Plus Protein™ Dual Color standards are a mixture of ten recombinant proteins （10–250 kD）, including eight blue-stained bands and two pink reference bands （25 and 75 kD）.
雙染蛋白MARKERPrecision Plus Protein™ Dual Color Stand
Applications and Uses
Choose Precision Plus Protein Dual Color standards for SDS-PAGE:
- Monitoring electrophoretic separation
- Molecular weight sizing
- Monitoring blot transfer efficiency with easy orientation via multicolor standards
- Fluorescent blot detection, with blue bands visualized in the red wavelength range and pink bands in the green wavelength （prepare a 1:10 dilution of the Dual Color standard and load 5 µl per well）
More Information
Loading buffer:
30% （w/v） glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN3, 0.01% bromophenol blue
