人宮頸癌細胞-HeLa細胞
人宮頸癌細胞-HeLa細胞是一個來自人體組織經(jīng)連續(xù)培養(yǎng)獲得的非整倍體上皮樣細胞系，它由GeyGO等在1951年從31歲女性黑人的宮頸癌組織建立。已知該細胞系含有人HPV病毒HPV18序列，需在2級生物安全防護臺操作。該細胞角蛋白陽性，p53表達量較低，但表達正常水平的pRB（視網(wǎng)膜母細胞瘤抑制因子）。
細胞名稱：人宮頸癌細胞
細胞簡稱：HeLa 細胞
細胞別稱：HELA; Hela; He La; He-La; Henrietta Lacks cells; Helacyton gartleri
產(chǎn)品貨號：TCH-C193
規(guī)格：1×10?cells， T25 培養(yǎng)瓶
種屬來源：人
組織來源：宮頸
特征：宮頸癌
細胞形態(tài)：上皮細胞樣
生長特性：貼壁生長
培養(yǎng)體系：MEM+10%FBS+1%P/S
人宮頸癌細胞-HeLa細胞 配套培養(yǎng)基貨號： TCH-G193
傳代比例：1:2-1:6，每2-3天換液一次
傳代周期：48-72 h
培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃
凍存條件：60%基礎培養(yǎng)基+30%FBS+10%DMSO，液氮儲存
質量檢測：細菌、真菌、支原體檢測均為陰性
實驗室儀器：二氧化碳培養(yǎng)箱
實驗耗材：T25細胞培養(yǎng)瓶，T75培養(yǎng)瓶,錐形瓶
參考文獻：
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Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217
Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 1648218
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