MRC-5細(xì)胞
細(xì)胞名稱：人胚肺細(xì)胞
細(xì)胞簡(jiǎn)稱：MRC-5
產(chǎn)品貨號(hào)：TCH-C263
種屬來源：人
組織來源：肺
疾病特征：/
細(xì)胞形態(tài)：成纖維細(xì)胞樣
生長(zhǎng)特性：貼壁生長(zhǎng)
培養(yǎng)體系： DMEM-H+10%FBS+1%P/S
配套培養(yǎng)基貨號(hào)：TCH-G263
傳代比例：1:3-1:6，每2-3天換液一次
傳代周期：48-72 h
培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃
凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲(chǔ)存
質(zhì)量檢測(cè)：細(xì)菌、真菌、支原體檢測(cè)均為陰性
MRC-5細(xì)胞是一種常用的人類胚肺細(xì)胞，是由1966年從一個(gè)正常的男性胎兒的肺部組織獲得，具有非常良好的細(xì)胞生長(zhǎng)和增殖特性，可廣泛應(yīng)用于細(xì)胞培養(yǎng)、病毒研究和毒性測(cè)試等領(lǐng)域。
該細(xì)胞系來自14周齡男性胎兒的正常肺組織，該細(xì)胞老化前能傳代42~46個(gè)倍增時(shí)間。
參考文獻(xiàn)：
Jacobs JP, et al. Characteristics of a human diploid cell designated MRC-5. Nature 227: 168-170, 1970. PubMed: 4316953
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Holliday R, Tarrant GM. Altered enzymes in ageing human fibroblasts. Nature 238: 26-30, 1972. PubMed:
Lewis CM, Tarrant GM. Error theory and ageing in human diploid fibroblasts. Nature 239: 316-318, 1972. PubMed:
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Kaur A, et al. Cytotoxic T-lymphocyte responses to cytomegalovirus in normal and simian immunodeficiency virus-infected Rhesus macaques. J. Virol. 70: 7725-7733, 1996. PubMed: 8892893
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Biological evaluation of medical devices--Part 5: Tests for in vitro cytotoxicity. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 10993-5:1999.
Standard Test Method for Determining the Virus-Eliminating Effectiveness of Liquid Hygienic Handwash and Handrub Agents Using the Fingerpads of Adult Volunteers. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1838-02.
Standard Test Method for Evaluation of Handwashing Formulation for Virus-Eliminating Activity using the Entire Hand. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2011-99.
Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2197-02.
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