CoverWell™ imaging chamber gaskets produce a chamber 20 mm in diameter and 1.0 mm deep. CoverWell imaging chamber gaskets incorporate a thin, optically clear plastic cover, making them ideal for light, epifluorescence and confocal laser-scanning microscopy. By simply pressing an imaging chamber gasket to a microscope slide or coverslip, a sealed chamber is formed to containing mounting medium. The watertight chamber supports and stabilizes thick and free-floating specimens permitting resolution of fine internal structures and analysis of markers without the compression or movement artifacts that affect observations made using an ordinary coverslip.