Anti-Brain2抗體,大腦蛋白2抗體

英文介紹：The Brn family of transcription factors are found in a highly restricted subset of neurons and are critical to the early embryonic development of the central nervous system. Brn-1 and Brn-2 are class III POU domain proteins. Expressed during the development of the forebrain and coexpressed in most layer II-V cortical neurons, Brn-1 and Brn-2 appear to critically control the initiation of radial migration of cortical neurons. Brn-2 is thought to be involved in smooth muscle cell development and differentiation. Brn-3 is a class IV POU domain protein. Three Brn-3 proteins have been described and are designated Brn-3a, Brn-3b and Brn-3c. Brn-3a has two functional transactivating domains, one at the amino terminus and one at the carboxy terminus. While Brn-3a and Brn-3c stimulate transcription, Brn-3b generally functions as a transcriptional repressor. However, Brn-3b, but not Brn-3a, has been shown to regulate the expression of the acetylcholine receptor.
此產(chǎn)品本公司有標記的一抗出售，相關(guān)標記有：Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

產(chǎn)品名稱：Anti-Brain2抗體,大腦蛋白2抗體

產(chǎn)品編號：BYK-10077R
英文名稱：Anti-Brain2
中文名稱：大腦蛋白2抗體
英文別名：Brain 2; Brain specific homeobox/POU domain protein 2; Brain-2; Brain-specific homeobox/POU domain protein 2; BRN 2; Brn-2; BRN2; N Oct 3; N OCT 3 Gene; Nervous system specific octamer binding transcription factor; Nervous system-specific octamer-binding transcription factor N-Oct-3; Oct-7; OCT7; Octamer Binding Transcription Factor 7; Octamer-binding protein 7; Octamer-binding transcription factor 7; OTF 7; OTF-7; OTF7; PO3F2_HUMAN; POU class 3 homeobox 2; POU domain class 3 transcription factor 2; POU domain, class 3, transcription factor 2; Pou3f2; POUF 3; POUF3; Protein Brn 2.
產(chǎn)品規(guī)格：0.1ml/0.2ml
此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯(lián)免疫(ELISA)，IF、IP、流式細胞術(shù)等實驗（可來電索取說明）
保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-Brain2抗體,大腦蛋白2抗體產(chǎn)品現(xiàn)貨銷售，其他同類產(chǎn)品：

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byk-11309R    精形成相關(guān)凋亡蛋白2抗體    Anti-TSARG2
byk-1309R    多配體蛋白聚糖1抗體    Anti-CD138/Syndecan 1
byk-2449R    腫瘤壞死因子受體超家族成員9抗體    Anti-CD137
byk-12449R    乙醇脫氫酶6抗體    Anti-ADH6
byk-10395R    金屬蛋白酶組織抑制因子-2抗體    Anti-TIMP-2
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byk-1738R    磷酸化原基因c-kit抗體    Anti-phospho-c-kit(Tyr936)
byk-0672R    干細胞生長因子受體/細胞表面分化抗原抗體    Anti-CD117/c-kit/SCFR
byk-0579R    CD105抗體    Anti-CD105/Endoglin
byk-0527R    CD10抗體    Anti-CD10
byk-11780R    指甲髕骨綜合征相關(guān)蛋白NPS1抗體    Anti-LMX1b/NPS1
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byk-11710R    脊髓小腦共濟失調(diào)蛋白BEAN1抗體    Anti-BEAN1/SCA31
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Anti-Brain2抗體,大腦蛋白2抗體Western Blotting 具體實驗步驟：

Western，也稱Western blot、Western blotting、Western 印跡，是用抗體檢測蛋白的重要方法之一。
Western可以參考如下步驟進行操作：
收集蛋白樣品(Protein sample preparation)
可以使用適當?shù)牧呀庖毫呀赓N壁細胞、懸浮細胞或組織樣品。對于某些特定的亞細胞組份蛋白，例如細胞核蛋白、細胞漿蛋白、線粒體蛋白等，可以參考相關(guān)文獻提取這些亞細胞組份蛋白，也可以使用試劑盒進行抽提。
收集完蛋白樣品后，為確保每個蛋白樣品的上樣量*，需要測定每個蛋白樣品的蛋白濃度。根據(jù)所使用的裂解液的不同，需要采用適當?shù)牡鞍诐舛葴y定方法。因為不同的蛋白濃度測定方法對于一些去垢劑和還原劑等的兼容性差別很大。

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